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Benson's Microbiological Applications Complete Version

© 2012
by Alfred Brown

12th Edition Active, In-Print 576 Pages Softcover
9780077302139 0077302133

Benson's Microbiological Applications has been the gold standard of microbiology laboratory manuals for over 30 years. The 77 self-contained, clearly-illustrated exercises, and four-color format makes Microbiological Applications: Laboratory Manual in General Microbiology, the ideal lab manual. Appropriate for either a majors or non-majors lab course, this lab manual assumes no prior organic chemistry course has been taken.

Part 1 Microscopy

1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Fluorescence Microscopy

5 Microscopic Measurements

Part 2 Survey of Microorganisms

6 Protozoa, Algae and Cyanobacteria

7 Ubiquity of Bacteria

8 The Fungi: Molds and Yeasts

Part 3 Manipulation of Microorganisms

9 Aseptic Technique

10 Pure Culture Techniques

Part 4 Staining and Observations of Microorganisms

11 Smear Preparation

12 Simple Staining

13 Negative Staining

14 Capsular Staining

15 Gram Staining

16 Spore Staining: Two Methods

17 Acid-Fast Staining: Kinyoun Method

18 Motility Determination

Part 5 Culture Methods

19 Culture Media Preparation

20 Preparation of Stock Cultures

21 Enumeration of Bacteria: The Standard Plate Count

22 Slide Culture: Fungi

Part 6 Bacterial Viruses

23 Determination of a Bacteriophage Titer

24 Burst Size Determination: A One-Step Growth Curve

25 Isolation of Phage from Flies

26 Phage Typing

Part 7 Environmental Influences and Control of Microbial Growth

27 Effects of Oxygen on Growth

28 Temperature: Effects on Growth

29 pH and Microbial Growth

30 Water Activity and Osmotic Pressure

31 Ultraviolet Light: Lethal Effects

32 The Effects of Lysozyme on Bacterial Cells

33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic

34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

35 Evaluation of Antiseptics: The Filter Paper Disk Method

36 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

37 Morphological Study of An Unknown Bacterium

38 Cultural Characteristics

39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions

40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions

41 Physiological Characteristics: Multiple Test Media

42 Use of Bergey's Manual

Part 9 Miniaturized Multitest Systems

43 Enterobacteriaceae Identification: The API 20E System

44 Enterobacteriaceae Identification: The Enterotube II System

45 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System

46 Staphylococcus Identification: The API Staph System

Part 10 Diversity and Environmental Microbiology

47 Isolation of an Antibiotic Producer: The Actinomyces

48 Nitrogen Cycle: Ammonification

49 Symbiotic Nitrogen Fixation: Rhizobium

50 Free-Living Nitrogen Fixation: Azotobacter

51 Denitrification: Paracoccus denitrificans

52 The Winogradsky Column

53 Purple Nonsulfur Photosynthetic Bacteria

54 Sulfate Reducing Bacteria: Desulfovibrio

55 Bacterial Commensalism

56 Bacterial Synergism

57 Microbial Antagonism

Part 11 Applied Microbiology

58 Bacterial Food Counts

59 Bacteriological Examination of Water: Most Probable Number Determination

60 Bacteriological Examination of Water: The Membrane Filter Method

61 Reductase Test

62 Temperature: Lethal Effects

63 Microbial Spoilage of Canned Food

64 Microbiology of Alcohol Fermentation

Part 12 Bacterial Genetics and Biotechnology

65 Mutant Isolation by Replica Plating

66 Bacterial Transformation

67 Polymerase Chain Reaction for Amplifying DNA

68 Plasmid Isolation

Part 13 Medical Microbiology

69 The Staphylococci: Isolation and Identification

70 The Streptococci and Enterococci: Isolation and Identification

71 Gram-Negative Intestinal Pathogens

72 A Synthetic Epidemic

Part 14 Immunology and Serology

73 Slide Agglutination Test: Serological Typing

74 Slide Agglutination Test for S. aureus

75 Slide Agglutination Test for Streptococcus

76 Tube Agglutination Test: The Heterophile Antibody Test

77 Blood Grouping Appendices

New to this Edition

Part 14 " Immunology and Serology. Select exercises have been removed from Medical Microbiology and Immunology and moved to this new part: Slide Agglutination Test: Serological Typing; Slide Agglutination Test for S. aureus; Slide Agglutination Test for Streptococcus; Tube Agglutination Test: Heterophile Antibody Test; and Blood Grouping.

Exercise 75: Slide Agglutination Test for Streptococcus.

Two exercises: Slime Mold Culture and White Blood Cell Study: The Differential WBC Count have been moved online to the website for easy reference and printing. Contact your sales rep for access information.

Many of the exercises have been thoroughly revised and updated, including Exercise 27 (Effects of Oxygen on Growth), Exercise 61 (Reductase Test for Milk), Exercise 63 (Microbial Spoilage of Canned Food), Exercise 69 (The Staphylococci: Isolation and Identification), Exercise 70 (The Streptococci and Enterococci: Isolation and Identification), Exercise 71 (Gram-Negative Intestinal Pathogens), and Exercise 72 (A Synthetic Epidemic). All exercises are up-to-date, informative, and relevant for any introductory microbiology laboratory. Many of the laboratory report questions have been revised as well.

To purchase an eBook version of this title, visit www.CourseSmart.com (0077507282). With the CourseSmart eTextbook version of this title, students can save money, reduce their impact on the environment, and access powerful web tools for learning. Faculty can also review and compare the full text online without having to wait for a print desk copy.

New! Connect Microbiology for Brown 12/e allows instructors and students to use art and animations from the text for assignments and lectures. Instructors now have access to a variety of new resources including assignable and gradable pre- and post-lab questions from the lab manual, additional pre- and post-lab activities, lab skill videos and more. In addition, digital images, PowerPoint slides and instructor resources are available through Connect.

Digital Lecture Capture: Tegrity Campus is a service that allows class time to be any time by automatically capturing every lecture in a searchable video format for students to review at their convenience. With a simple one-click process, you can capture all computer screens and corresponding audio. Students may replay any part of your class with simple browser-based viewing on a PC or Mac. Educators know that the more students can see, hear, and experience class resources, the better they learn. Help turn all your students? study time into learning moments by supplying them with your lecture videos.

Customize this book through McGraw-Hill Create! You can customize this book to meet your exact needs and mix and match with other texts on Create--allowing you maximum choice and flexibility. www.mcgrawhillcreate.com

Learning outcomes for each exercise. Students and instructors will be able to assess if the purpose of the laboratory exercise has been accomplished and understood.

Retained Features

Basic microbiology safety rules are included in the front matter. These rules and regulations provide the information required to assure a safe learning environment.

The gold standard in microbiology lab manuals incorporates many features for ease-of-use, including: user-friendly diagrams that students can easily follow, clear instructions, and an excellent array of reliable exercises suitable for beginning or advanced microbiology courses. Laboratory reports are designed to guide and reinforce student leaning and provide a convenient place for recording data.

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